# Hak_acclim # The harmful alga Heterosigma akashiwo (CCMP2393) grown under a range of CO2 concentrations from 2 P1000 ppm. # PI's: S. Dyhrman (LDEO), J. Morris (U Alabama) # version: 2018-10-11 # See also: https://www.ncbi.nlm.nih.gov/bioproject/377729 # ========================= sample_name sample_title bioproject_accession organism strain isolate host isolation_source collection_date geo_loc_name sample_type biomaterial_provider collected_by depth env_biome genotype lat_lon passage_history samp_size temp_C light_level_umol_m2_s light_dark_hr Media CO2_ppm Alkalinity pH ------------------------- SS0095 RNAseq of H. akashiwo premodern CO2 A PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-07-13 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 206.64 2096 8.14 SS0096 RNAseq of H. akashiwo premodern CO2 B PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-06-21 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 303.65 2037 8.00 SS0097 RNAseq of H. akashiwo premodern CO2 C PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-06-21 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 284.83 2077 8.03 SS0098 RNAseq of H. akashiwo modern CO2 A PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-07-13 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 474.76 2089 7.85 SS0099 RNAseq of H. akashiwo modern CO2 B PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-06-21 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 330.88 2089 7.97 SS0100 RNAseq of H. akashiwo modern CO2 C PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-07-13 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 462.09 2079 7.86 SS0101 RNAseq of H. akashiwo RCP4.5 CO2 A PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-07-13 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 538.82 2083 7.79 SS0102 RNAseq of H. akashiwo RCP4.5 CO2 B PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-07-13 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 493.47 2063 7.82 SS0103 RNAseq of H. akashiwo RCP4.5 CO2 C PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-06-21 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 681.63 2029 7.70 SS0104 RNAseq of H. akashiwo RCP8.5 CO2 A PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-06-21 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 1051.43 2082 7.54 SS0105 RNAseq of H. akashiwo RCP8.5 CO2 B PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-06-21 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 1033.74 2044 7.54 SS0106 RNAseq of H. akashiwo RCP8.5 CO2 C PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-06-21 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 966.39 2087 7.57 SS0107 RNAseq of H. akashiwo RCP6 CO2 A PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-06-21 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 821.79 2079 7.63 SS0108 RNAseq of H. akashiwo RCP6 CO2 B PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-06-21 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 823.69 2076 7.63 SS0109 RNAseq of H. akashiwo RCP6 CO2 C PRJNA377729 Heterosigma akashiwo CCMP2393 nd nd nd 2017-06-21 USA: Lamont Doherty Earth Observatory cell culture NCMA nd nd coastal temperate ocean nd nd acclimated for 3 days at respective CO2 concentration ~6 x 10^6 cells 18 100 14:10 L1 medium (without silicate) made with a Long Island Sound seawater base collected from Avery Point, CT, USA (salinity 32) 694.17 2089 7.70